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Effect of XF-iMSCs-secreted EVs in mouse <t>primary</t> <t>cells.</t> <t>XF-iMSC-secreted</t> EVs <t>(XF-iEv)</t> and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).
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Effect of XF-iMSCs-secreted EVs in mouse <t>primary</t> <t>cells.</t> <t>XF-iMSC-secreted</t> EVs <t>(XF-iEv)</t> and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).
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Effect of XF-iMSCs-secreted EVs in mouse <t>primary</t> <t>cells.</t> <t>XF-iMSC-secreted</t> EVs <t>(XF-iEv)</t> and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).
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Effect of XF-iMSCs-secreted EVs in mouse primary cells. XF-iMSC-secreted EVs (XF-iEv) and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).

Journal: Regenerative Therapy

Article Title: Anti-inflammatory and immunomodulatory effects of human induced pluripotent stem cells-derived mesenchymal stem/stromal cells and their extracellular vesicles

doi: 10.1016/j.reth.2026.101081

Figure Lengend Snippet: Effect of XF-iMSCs-secreted EVs in mouse primary cells. XF-iMSC-secreted EVs (XF-iEv) and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50). Data are presented as mean ± SD. + p < 0.05, ++ p < 0.01, +++p < 0.001 (Aspin-Welch's t -test), # p < 0.05; ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).

Article Snippet: XF-iMSC-secreted EVs (XF-iEv) and human adipocyte-derived MSC-secreted EVs (hAC-Ev) were purified using the MagCapture Exosome Isolation Kit. (A) Mouse peripheral blood mononuclear cells (mPBMCs; 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h. Levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. (B) Mouse splenocytes (2 × 10 6 cells/mL) were stimulated with Dynabeads CD3/CD28 for 24 h. Levels of IL-2, IFN-γ, and IL-17 in culture supernatants were quantified by ELISA. hPBMCs were cultured with different concentrations of XF-iEv or hAC-Ev (x1, x2, x10, x50).

Techniques: Derivative Assay, Purification, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture, Control